A comparison of gas stream and plunge cooling in macromolecular cryocrystallography
نویسندگان
چکیده
منابع مشابه
Macromolecular Cryocrystallography: Cooling, Mounting, Storage and Transportation of Crystals
Simple methods are presented for handling, mounting, storage and transportation of crystals at cryogenic temperatures. They are easy to learn and have a number of technical and operational advantages over currently popular methods. In particular, the temperature of the crystal throughout all manipulations is known; it is shown never to rise above that of the warmest component of the cryogenic s...
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The current techniques for X-ray-diffraction data collection from macromolecular crystals at cryogenic temperatures are reviewed. The development of the experimental methods is outlined and the basic concepts pertaining to radiation damage and cryoprotection are summarized. Emphasis is placed on the practical aspects important to the success of the techniques, and a detailed account of these is...
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Cryocrystallography is an indispensable technique that is routinely used for single-crystal X-ray diffraction data collection at temperatures near 100 K, where radiation damage is mitigated. Modern procedures and tools to cryoprotect and rapidly cool macromolecular crystals with a significant solvent fraction to below the glass-transition phase of water are reviewed. Reagents and methods to hel...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations and Advances
سال: 2018
ISSN: 2053-2733
DOI: 10.1107/s010876731809596x